Effects of Carpio Decoction on the Structure of Kidney in Rats with Adriamycin-Induced Nephrosis

The aim of this study was to observe the effects of Cyprinus carpio decoction on the expression of aquaporins in rats with adriamycin-induced nephropathy and to explore the therapeutic mechanism on nephrotic edema. Total of 50 Wistar rats were randomly divided into normal group, model group, fosinopril group, Cyprinus carpio decoction treated with high dose group and low dose group consisting of 10 rats respectively. Nephropathy models were established by injecting adriamycin through tail vein and treated with Cyprinus carpio decoction. Urinary protein excretions in 12 h, serum albumin, total serum protein, serum sodium and potassium were measured by biochemical assay. The pathological changes and the expression of AQP1, AQP2, AQP3 in rat kidneys were respectively detected by HE stain and immunohistochemiscal assay. The results indicated: 1) The urinary protein excretion in 12 h (proteinuria) increased significantly along the time longed modeling, while no significant increasing in Cyprinus carpio decoction treated group (F = 5.23 41.89, P < 0.05); 2) The serum albumin and total protein in model group were significantly lower than that in normal group, but that in Cyprinus carpio decoction treated group were higher than that in model group (F = 13.12 15.48, P < 0.05). The serum sodium and potassium in model group were higher than those in normal group, while that in Cyprinus carpio decoction treated group were higher than that in model group (F = 3.42 3.96, P < 0.05); 3) Renal glomerular capillaries congestive of group M rats were expansion. Glomerular mesangial cells and the basement membrane were diffuse hyperplasia, inflammatory cell infiltration. Glomerular mesangial cells and the basement membrane of Cyprinus carpio decoction with interventing groups reduced proliferation, less inflammatory cell infiltration; 4) In model group, the expressions of AQP1-3 in the renal tubule and collecting duct cells increased significantly than those in normal group, and those in Cyprinus carpio decoction treated group decreased than those in model group (F = 3.97 6.19, P < 0.05). It is suggested that Cyprinus carpio decoction could reduced the urinary protein excretion and alleviate pathological lesion and edema with adriamycin-induced nephropathy by decreasing the expressions of AQPs in kidneys.